Fig. 4. Mlp84B does not have an essential nuclear function. (A-C) Two muscle fibers from transgenic third-instar larvae expressing either an (A) Mlp84B::GFP, (B) Mlp84B::GFP::NES or (C) Mlp84B::GFP::NLS fusion protein. Muscle nuclei in A and B are highlighted by red circles. Note the lack of detectable nuclear GFP signal in B relative to A and the strong nuclear signal in C. The three micrographs were recorded using the same confocal settings. (D) Quantification of rescue of the mlp84B^P8 mutants by the three Mlp84B fusion proteins. The Mlp84B::GFP::NLS does not provide any appreciable rescue of pupal lethality in the mlp84B^P8 mutants.