Fig. 2. Nucleolin depletion causes disorganization of nucleoli. (A) Immunoblot showing effective depletion of nucleolin by two siRNAs. Fibrillarin, B23,
-tubulin and β-actin are shown as controls. (B) Quantification of nucleolin levels in the nucleoli and chromosome periphery evaluated by immunostaining. Values represent the mean of three independent experiments ± s.d. (n=10). (C) Interphase cells stained for nucleolin (green) and fibrillarin (red). (D) Interphase cells stained for B23 (green). (E) Interphase cells stained for Ki-67 (green) and fibrillarin (red).