Fig. 4. Nucleolin depletion induces defects in chromosome congression. (A) Mitotic cells stained with an anti-nucleolin antibody. The misalignment (i) and non-alignment (ii) phenotypes are shown. (B) Metaphase chromosome spreads stained with nucleolin (green) and fibrillarin (red) antibodies. (C) Mitotic cells stained with -tubulin (green) and CREST (red) antibodies. The misalignment (i) and non-alignment (ii) phenotypes are shown. Arrowheads show misaligned chromosomes and the fluorescence signals for CREST at the kinetochores. Bars, 5 µm. (D) Quantification of the distributions of chromosome congression defects following transfections. Values represent the mean of four independent experiments (n=400). (E) Histogram showing the numbers of chromosomes that fail to align in each cell. Values represent the mean of four independent experiments ± s.d. (n=400).