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Figure 2


Fig. 2. Co-immunoprecipitation confirms the unique and specific interaction between {alpha}T-catenin and PKP2 both in vitro and in vivo. (A) Myc-tagged {alpha}T-catenin (114 kDa, left panel), Myc-tagged {alpha}E-catenin (111 kDa, right panel) and E-tagged full-length human PKP2 (100 kDa) were produced by coupled transcription-translation in vitro. Proteins immunoprecipitated with anti-Myc or anti-E-tag antibodies were analyzed by western blot. Interaction between {alpha}T-catenin and PKP2 is clearly observed (left panel; asterisk), but no interaction is seen between {alpha}E-catenin and PKP2 (right panel; arrowhead). (B) Endogenous PKP2 was co-immunoprecipitated with {alpha}T-catenin from mouse heart lysate. {alpha}T-catenin was specifically immunoprecipitated with polyclonal antibody 952, followed by western blot analysis using a PKP2-specific monoclonal antibody.