Fig. 10. Skeletor distribution in crane-fly and locust spermatocytes. (A) A prophase cell illustrating staining of chromosomes, the cell cortex, the asters, the polar flagella (arrow indicates one flagellum) and the poles (asterisk indicates one pole). (B) An early anaphase cell, illustrating staining along the kinetochore and non-kinetochore spindle fibers, defining the spindle area. Asterisks indicate the two poles. (C) A telophase/cytokinesis cell illustrating staining of the mid-body region. (D) A prometaphase cell stained for skeletor (green) and tubulin (red). Skeletor and tubulin co-localize (yellow-orange) mainly along kinetochore spindle fibers and at the poles. (E) Enlarged detail of a kinetochore spindle fiber and a bivalent at metaphase (slice 24 of the same cell depicted in Fig. 12), showing the beaded distribution of skeletor along these spindle fibers and around the chromosomes. (F) A prophase locust spermatocyte illustrating skeletor staining of the chromosomes, their periphery (arrowheads) and the cell cortex. (G) An anaphase locust spermatocyte. Skeletor is seen as punctate regions in the chromosomes, along the kinetochore spindle fibers, along the interzone connections between separating half-bivalents, and in high concentration at the poles. (H) Enlarged detail of two consecutives confocal slices (merged) of the same cell as in G, illustrating the interzone connections (arrowheads) between separating half-bivalents. (I) A telophase cell. Skeletor is present in high concentration in the mid-body, in the contractile ring (arrowhead) and in the newly formed nuclear membrane (arrows). (J) Metaphase locust spermatocyte double stained for skeletor (green) and tubulin (red). Skeletor and tubulin co-localize (yellow-orange) mainly along kinetochore spindle fibers and at the poles, similar to the pattern observed in crane-fly spermatocytes. Bars, 5 µm.