Fig. 6. D-titin distribution in anaphase and telophase insect spermatocytes. (A,B) Distribution of anti-KZ, anti-52 and anti-56 antibodies against D-titin (green) in anaphase crane-fly spermatocytes (A) and anaphase locust spermatocytes (B). (C,D) Distribution of tubulin (red) in crane-fly spermatocytes (C) and locust spermatocytes (D). (E,F) Merged channels of D-titin and tubulin. Insert in A, left panel, is an enlarged detail of the boxed region, showing punctate D-titin connections between the separating half-bivalents. (G,H) Distribution of anti-KZ, anti-52 and anti-56 antibodies against D-titin in telophase crane-fly spermatocytes (G) and in telophase locust spermatocytes (H). D-titin is associated with mid-body microtubules, with chromatin, with the nascent nuclear membranes (arrows in G, left and right panel) and with the flagella (arrowhead in G, third panel). Bars, 5 µm.