Fig. 8. Distribution of anti-KZ antibody compared with myosin distribution in insect spermatocytes. (A) Merged channels of D-titin (green) and myosin (red) staining in metaphase (left panel) and anaphase (right panel) crane-fly spermatocytes (Z-series), showing their co-localization (yellow-orange) in the chromosomes (arrow), at the poles (arrowheads) and along the spindle fibers. (B) Enlarged detail of the boxed region in A showing the side-by-side distribution of D-titin (green) and myosin (red) in the interzone along the connections between separating half-bivalents (open arrowheads). Arrows indicate the two sex chromosomes. (C) Merged channels of D-titin (green) and myosin (red) staining in metaphase (left panel) and anaphase (right panel) locust spermatocytes, showing their co-localization (yellow-orange) at the poles (arrowheads) and along the spindle fibers. The arrow in the left panel indicates the sex chromosome and the arrows in the right panel indicate two autosomal half bivalents. Open arrowheads indicate side-by-side distribution of D-titin and myosin staining in the interzone along the connections between separating half-bivalents. Bars, 5 µm.