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Figure 3


Fig. 3. Nuclei determine position of branches. (A) Quantification of distance between nucleus and branch in individual cdc25-22-blocked cells 4 hours after centrifugation in the presence of MBC (black) or without centrifugation (grey). 82 cells were measured. DAPI staining of branching cdc25-22-blocked cells in MBC after centrifugation (lower panel). (B) Scoring of branching in cdc25-22- and cdc25-22 mto1{Delta}-blocked cells in the presence of either DMSO or MBC. Prior to treatment cells were blocked for 3 hours at 36.5°C. (C) Branching cdc11-119 cells treated with DMSO or MBC over a 5-hour time course. Cells were blocked at the restrictive temperature for 4 hours prior to drug treatment. (D) Images of DAPI-(top) and actin (bottom)-stained cdc11-119 cells blocked at 36.5°C for 4 hours and then treated with MBC for 6 hours. Bar, 5 µm.