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Figure 4


Fig. 4. Tea1 accumulates in the middle of the cell in the absence of microtubules. (A) Anti-Tea1 antibody staining of cdc25-22 cells blocked for 3 hours at 36.5°C and treated with DMSO (top) or MBC for 30, 45 and 60 minutes (below). Cells were fixed in TCA to allow visualization of Tea1 in the cell middle. Tea1 staining in the cell centre is lost with methanol fixation. (B) Brightfield images of cdc25-22 mid1{Delta} cells blocked for 3 hours at 36.5°C and then for 1 hour in 50 µg/ml MBC. Bar, 5 µm.