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Figure 8


Fig. 8. Hypoxia-mediated translocation of PKC{zeta} is independent of RhoA. Serum-starved WT and DN-RhoA-A549 cells were exposed to 21% O2 or 1.5% O2 for 20 minutes. Triton X-100-soluble membrane fractions were obtained and PKC{zeta} translocation was evaluated by western blot. Top: bars represent mean ± s.e.m. (n=3). **P<0.01. Bottom: a representative western blot for PKC{zeta} translocation and total {alpha}1-Na,K-ATPase as loading control.