Fig. 2. (A,C) Tether force vs tether growth velocity at room temperature (A) and 37°C (C). The solid lines are linear fits to the corresponding data sets (control, ; depletion, ; enrichment, ). Inset in A, cholesterol level measured by GLC (see Materials and Methods). ctrl, control; dpl, cholesterol depleted; enrch, cholesterol enriched. The values of slopes (_eff) and intercepts (F₀) are listed in Table 1. (B,D) Bar graph for F₀ at room temperature (B) and 37°C (D). Error bars in A and C represent s.e. obtained from the experimental data points. Errors in the bar graphs represent the error of the linear fit to the data. All six R² values were larger than 0.97.