Fig. 8. The distribution of occludin and ZO-1 in EpH4 cells at various times after plating determined by dual label immunohistochemistry. Occludin and ZO-1 staining of the same region are shown separately in black and white images. Images were taken in cultures fixed after plating (subconfluent) and 24 and 72 hours later in the presence and absence of hydrocortisone (+ or –HC, respectively).