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Figure 4


Fig. 4. aPKC{lambda} inhibition by RNAi mimics the effect of PAR6{Delta}N on apoptosis. (A) MDCK cells transfected with either an empty vector (control) or pSuper-aPKC{lambda} RNAi were grown on Matrigel for 3 days and subjected to immunoblotting with total-aPKC{lambda} (upper panel) and GAPDH (lower panel). (B) Control and aPKC{lambda} RNAi MDCK cells grown on Matrigel were immunostained with aPKC{lambda} (green; left), ZO-1 (white; middle) and cleaved caspase-3 (red; right). Bar, 10 µm. (C) Percentage of apoptotic cells was evaluated by cleaved caspase-3 staining comparing control and aPKC{lambda} RNAi MDCK cells. Results are the mean±s.d. of three experiments.