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Figure 1


Fig. 1. Endocytosis of membrane and fluid. Ax2 cells, grown in axenic medium, were washed free of growth medium and used immediately (vegetative cells) or starved for approximately 5 hours with periodic cAMP pulses after the first hour (aggregation competent cells). (A) Aggregation-competent cells stained with FM1-43: after a few seconds labelling is restricted to the cell surface, but by 100 seconds, there is additional punctate labelling within the cell, presumably as a result of endocytosed membrane (confocal sections). (B) Membrane endocytosis by vegetative ({square}) and aggregation-competent ({circ}) cells determined using FM1-43, with 10 mM azide added as indicted (Figure 1, Figure 1). Results are given as the fraction of the cell surface taken up by normalizing to the first measurement after mixing dye and cells. Inset shows a continuous trace made by adding FM1-43 directly to the cuvette illustrating the nearly instantaneous increase in fluorescence. (C) Fluid-phase endocytosis by vegetative ({square}) and aggregation-competent cells ({circ}) determined using FITC dextran. Values are mean ± s.d. of three experiments.