Fig. 8. Surface area and volume of cells moving under buffer. Ax2 cells expressing soluble GFP were brought to aggregation-competence and filmed by taking confocal stacks every 9-13 seconds; the reconstructed cells are viewed at a 30° angle to the substratum. The surface area and volume of the cell are given above each image (A, V, respectively), normalized to the values at the start of the sequence.(A) A randomly moving cell (at 0 seconds the surface area=567 µm²; volume=547 µm³); (B) chemotaxing cell moving to the right towards a micropipette containing cAMP (506 µm²; 562 µm³); (C) a quiescent cell, stimulated with a micropipette containing cAMP (to the right) at the start of the sequence (397 µm²; 361 µm³); (D) a quiescent cell, not stimulated (211 µm²; 191 µm³). Bars, 15 µm. Selected time points are shown. See Movies 12-14 in supplementary material.