Fig. 3. Requirement of Par-3 for co-localization of afadin with nectin. (A) Reduction in the immunofluorescence signal for afadin at cell-cell adhesion sites in Par-3-knockdown MDCK cells. After Ca²⁺ switch, the cells were fixed and stained with various combinations of the indicated Abs. Bars, 10 µm. (B) Reduction in the immunofluorescence signal for afadin, but not that for nectin-1, at the nectin-based cell-cell adhesion sites in Par-3-knockdown nectin-1-MDCK cells. After Ca²⁺ switch, the cells were fixed and stained with various combinations of the indicated Abs. Bars, 10 µm.