Fig. 4. No requirement of afadin for co-localization of Par-3 with nectin. (A) Reduction in the amount of afadin by RNAi. The lysates of afadin-knockdown nectin-1-MDCK cells were subjected to western blotting with the indicated Abs. (B) Concentration of the immunofluorescence signals for Par-3 and nectin-1 at nectin-based cell-cell adhesion sites in afadin-knockdown nectin-1-MDCK cells. After Ca²⁺ switch, the cells were fixed and stained with various combinations of the indicated Abs. Bars, 10 µm.