Fig. 8. Cooperative actions of Par-3 and afadin in the formation of AJs and TJs. After Ca2+ switch, the Par-3-knockdown MDCK cells that expressed GFP-afadin were fixed and stained with various combinations of the indicated Abs. Bars, 10 µm. Asterisks indicate the Par-3-knockdown MDCK cells that expressed GFP-afadin.