Fig. 2. CMS colocalizes with podosomes. (A) NIH 3T3 cells ectopically expressing activated Src (Src^*3T3) were transduced by retroviral infection with vector and vector carrying CMS. Cells were stained for CMS and F-actin and analyzed for subcellular localization. Prominent rosette-like structures composed of individual podosomes are formed in Src^*3T3 cells (see arrows), and colocalization of CMS with these structures in Src^*3T3/CMS cells is apparent (see arrows). Bars, 20 µm. (B+C) Confocal immunofluorescence microscopy analysis of CMS and actin in Src^*3T3/CMS cells. (B) Images were taken at ventral and dorsal layers. Foot processes composed of podosomes are marked with arrowheads, lamellipodia are marked with an arrow. Bars, 20 µm. (C) Images of two cells taken at the ventral layer show prominent podosome clusters. Bars, 20 µm. Inset left: high-magnification z-axis images of individual podosomes show localization of CMS to the core; bar, 5 µm.