Fig. 3. Antibody uptake experiment to evaluate retrieval to the TGN. HeLa cells stably transfected with CD8-CIMPR and the various truncations were incubated with the monoclonal anti-CD8 continuously for 3 hours prior to fixation. Cells were labelled with anti-TGN46 followed by appropriate secondary antibodies. All the constructs can retrieve to the TGN with the exception of the 1-75 truncation. Bar, 20 µm.