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Figure 4


Fig. 4. The {Delta}1-75 truncation accumulates in VPS26-positive endosomes and is unstable. (A) Cells expressing the {Delta}1-75 truncation were incubated for three hours continuously with the anti-CD8 antibody prior to fixation. Cells were labelled with anti-VPS26 antiserum followed by appropriate secondary antibodies. There is significant colocalisation of the endocytosed anti-CD8 and VPS26 (shown enlarged in the inset boxes and indicated by arrows). Bar, 20 µm. (B) Cells expressing the CD8-CIMPR and truncations were treated with 100 µg/ml cycloheximide for 0, 3 or 6 hours. Cells were then lysed and the CD8 reporter immunoprecipitated and subjected to SDS-PAGE and western blotting. The {Delta}1-75 truncation is unstable and undetectable after 3 hours of cycloheximide treatment.