Fig. 6. Subcellular localization of polarity proteins in the tetO₇:ROT1 mutant strain. Exponentially growing cells of the tetO₇:ROT1 (JCY216) strain transformed with a plasmid expressing a GFP-tagged version of the Spa2 (A), Cdc24 (B) or Cdc42 (C) proteins were synchronized by addition of -factor in the presence of 5 µg/ml doxycycline. DIC images and GFP signal of a collection of cells at the different cell cycle stages are shown.