Fig. 9. Genetic interactions between the ROT1 gene and genes of the ubiquitin-proteasome pathway. (A) Tenfold serial dilutions from exponentially growing cultures of the wild type (W303 or WCG4 in the upper panel), tetO₇:ROT1 (MCY68 or MCY125 in the upper panel), pre1 pre2 (WCG4-11/22), pre1 pre2 tetO₇:ROT1 (MCY32), cdc16, cdc16 tetO₇:ROT1 (JCY553), apc2 (KTM200U) and apc2 tetO₇:ROT1 (JCY555) were spotted onto YPD medium with or without 0.5 µg/ml doxycycline (Dox) and incubated at 30° for 3 days. (B) Tenfold serial dilutions from exponentially growing cultures of the cdc20 and cdc20 tetO₇:ROT1 (MCY139) strains transformed with a centromeric plasmid containing the ROT1 gene as indicated, were spotted onto YPD medium with or without 5 µg/ml doxycycline and incubated at 28°C or 37°C for 3 days.