Fig. 6. Non-clustered GFP-Kv2.1-loopBAD channels can become trapped after crossing the cluster perimeter. Illustrated is a Qdot655-tagged channel entering a cluster (yellow arrow) and being transiently retained within the cluster perimeter for 112 seconds before escaping. Cluster perimeter crossing events were relatively rare, being observed in only 9 out of more than 80 Qdot tracks analyzed. See supplementary material Movie 2. The Qdot was imaged every 0.8 seconds.