Fig. 7. Phalloidin-stained actin filaments direct Kv2.1 surface cluster orientation. HEK cells expressing GFP-Kv2.1-loopBAD were formaldehyde fixed, permeabilized with Triton X-100 and stained with Alexa Fluor 594-labeled phalloidin. (A) GFP-Kv2.1-loopBAD expression pattern. (B) Phalloidin pattern. (C) The overlay of panels A and B.