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Figure 8


Fig. 8. Kv2.1 surface clusters correlate with an absence of cortical actin. HEK cells were co-transfected with mCherry-Kv2.1-loopBAD and GFP-actin and imaged without fixation. (A) The expression pattern of GFP-actin. (C) Localization of mCherry-Kv2.1-loopBAD overlaid with GFP-actin expression. (B,D) Enlargements of the boxed regions in A and C. The yellow arrow in D indicates that the Kv2.1 cluster perimeter did not always align with the border of the actin-free zone. Nonetheless, Kv2.1 clusters tend to form where actin is reduced.