Fig. 3. CDK11^p58 protein partially rescues the premature sister chromatid separation phenotype caused by CDK11 depletion. HeLa cells stably expressing GFP-tagged CDK11^p58 protein from the CDK11^p58-GFP cell line or the RNAi-resistant form of the CDK11^p58m-GFP cell line were transfected with CDK11 RNAi or control RNAi plasmids and analyzed 48 hours later by immunoblotting for expression of CDK11 proteins with P1C antibody (A) and for mitotic chromosome appearance and mitotic index (B). Giemsa-stained cells were analyzed for mitotic chromosome appearance (100 cells) and mitotic index (>1000 cells). Representative data from one experiment are shown. Chromosome appearance was categorized as in Fig. 2.