Fig. 7. Analysis of mitotic chromosomes in cells treated with Plk1, Sgo1 and CDK11 RNAi expression constructs. (A,B) Quantitation of the chromosome phenotypes from Plk1, Sgo1 and CDK11 RNAi cells in a representative experiment. HeLa cells were transfected with CDK11, Plk1, Sgo1, Plk1-CDK11, Sgo1-CDK11, Sgo1-Plk1 or control RNAi expression constructs. Mitotic spreads were prepared and Giesma stained 48 hours later. More than 100 mitotic cells were scored for chromosome appearance. (C) The appearance of characteristic mitotic chromosomes. Bar, 3 µm.