Fig. 1. Localization of GFP-centrin to centrosomes. Exponentially growing, non-synchronized CHO cells possess centrosomes revealed as two (A-C) or sometimes four (D-F) fluorescent dots with varying distance between them. Arrowheads indicate a small centrosome next to the mother centrosomes (G-I,I'). In addition to the centriole, GFP-tagged centrin labels the PCM, which appears morphologically varied, from a fuzzy indistinct mass (J) to discrete dots or lines (arrows, K-N). Areas outlined in I' and J' are seen in I and J at high magnification. Bars, 2 µm (A-N); 10 µm (I'-J').