Fig. 2. pten^– cells abnormally extend multiple pseudopods at the same time. Different symbols represent different pseudopods. (A,B) The orderly extension of pseudopods by two representative parental AX2 cells. Note that only one pseudopod expands at a time, and that a previous pseudopod usually retracts when a new pseudopod expands. (C,D) The overlapping expansion of multiple pseudopods by two representative pten^– cells. Both anterior and lateral pseudopods were reconstructed and volumes estimated at each time point using 3D-DIAS software (Wessels et al., 1998; Heid et al., 2005).