JCS -- Yasumoto et al. 120 (15): 2532 Figure IG8

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 8. GNL3L suppresses SRC-mediated transcriptional coactivation of ERR ${gamma}$ . (A) Using the same cell-based reporter system as described in Fig. 4, we show that the ERE-specific transcriptional activity in cells coexpressing ERR ${gamma}$ and SRC1 (8.0±0.3) is 1.7 times higher than that of the ERR ${gamma}$ -expressing sample (4.8±0.3). When coexpressed with the wild-type GNL3L (WT), this ERR ${gamma}$ and SRC1-mediated ERE-specific transcriptional activity is reduced by 55% and 70% compared with the sample expressing both ERR ${gamma}$ and SRC1 in a dose-dependent manner. This inhibitory effect of GNL3L on the SRC1-mediated coactivation of ERR ${gamma}$ requires the I-domain of GNL3L because deletion of this domain (dI) fails to suppress the transcriptional activity of ERR ${gamma}$ and SRC1 (P=0.17). (B) Using the same approach, we show that GNL3L can also suppress the coactivator function of SRC2 on the ERR ${gamma}$ -dependent transcriptional activity in a dose-dependent (54% reduction for 100 ng of GNL3L and 71% reduction for 200 ng of GNL3L) and I-domain-dependent (P=0.58) manner. Error bars represent the stand error of mean (± s.e.m.). ^***P<0.0001.