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Figure 1


Fig. 1. MDCK cells expressing Fc38-63 (A-C), Fc38-63L50A (D-F) or Fc38-63Y47A (G-I) were incubated with rat anti-Fc receptor antibodies for 30 minutes at 4°C and then shifted to 37°C for 45 minutes. Cells were then fixed, permeabilized and incubated with anti-rat IgG conjugated to Alexa Fluor-594 (A,D,G) and Alexa Fluor-488-phalloidin (B,E,H). The confocal images correspond to an xy-slice through the middle of the cells. Bars, 10 µm.