Fig. 5. MDCK cells expressing Fc38-63 were transfected with wild-type dynamin 2 (A-C) or the dynamin 2 (K44A) mutant (D-F) in a vector that also expresses EGFP. In each instance, the cells were incubated with anti-Fc receptor antibodies for 30 minutes at 4°C and then shifted to 37°C for 30 minutes. Cells were then fixed, permeabilized and incubated with anti-rat IgG conjugated to Alexa Fluor-594. Bars, 10 µm.