Fig. 5. Knockdown of RhoD impairs translocation of Fyn-GFP and Src-GFP palmitoylation mutant. cDNA from SYF^–/– cells expressing (A) Fyn-WT-GFP and an siRNA control or Fyn-WT-GFP and RhoD siRNA or (B) Src-GFP S3C/S6C and an siRNA control or Src-GFP S3C/S6C and RhoD siRNA or (C) Src-WT-GFP and an siRNA control or Src-WT-GFP and RhoD siRNA was reverse transcribed (left panels). Cells from the same experiment were plated for 6 hours in serum-containing medium then stained for anti-P-Y416-SFK (Texas Red secondary). Solid arrows indicate SFK at the cell periphery whereas broken arrows show SFK retained in the perinuclear region. Bars, 25 µm.