Fig. 3. (A,B) Histological analysis of the epidermis in the hair follicle and around the hair funnel orifice. (C) Using an antibody against the proliferation marker Ki67 only basal keratinocytes were stained in control mice. (D) In K14Cre⁺/Miz1^lox/lox mice, suprabasal cells were also positive for Ki67, correlating with an increase in the number of cell layers in the epidermis. (E-H) In these areas some suprabasal cells were not only positive for keratin 1 (E,F) but also for keratin 14 (H; green), which was restricted to basal keratinocytes in control mice (G, green). Furthermore, K14Cre⁺/Miz1^lox/lox mice had a thicker cornified layer positive for filaggrin (G,H; red). Note that the suprabasal cells of the epithelium from cyst-like structures (asterisks) also express K1, which is usually found in the interfollicular rather than in the intrafollicular epidermis (compare with Fig. 2H). Bar, 100 µm (A,B,E-H); 20 µm (C,D).