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Figure 2


Fig. 2. Transfection of Aiolos isoforms in mammalian cells. (A) Schematic representation of the five selected isoforms. (B) Western blot of COS-7 cells extracts transfected with the five Aiolos isoforms and developed with the mouse monoclonal anti-FLAG antibody (left) and a polyclonal anti-Aiolos antiserum (right). (C) Subcellular localization of Aiolos isoforms. Confocal images of COS-7 cells transfected with different Aiolos isoforms and labeled with antibody to Aiolos (green). Nuclear DNA was counterstained with DAPI (blue). Images of different subpopulations are included (indicated in Results).