Fig. 6. Analysis of the interaction of Aiolos isoforms with the promoter of SIRT1. (A) ChIP assays were performed with anti-FLAG, anti-Aiolos and anti-RNA pol II antibodies in COS-7 cells transfected with all the Aiolos isoforms studied in this work. Negative controls (No antibody, NAB) are also shown. Input and bound fractions are shown. In addition to the SIRT1 promoter, actin B gene promoter was used as a negative control. (B) Association of Aiolos to the SIRT1 promoter in B cells. ChIP assays in untransfected RAJI and B-PBL cells. Anti-Aiolos and anti-RNA pol II antibodies were used. Actin B was used as a negative control.