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Figure 7


Fig. 7. Transfection of Aiolos isoforms in B cells. (A) Subcellular localization of Aiolos isoforms. Confocal images of RAJI and B-PBL cells transfected with different Aiolos isoforms and labeled with Aiolos antibody (green). Nuclear DNA was counterstained with DAPI (blue). Images of different subpopulations are included (indicated in Results). (B) Association of Aiolos isoforms with histone deacetylase. Confocal images of RAJI and B-PBL cells transfected with different Aiolos isoforms. Aiolos isoforms were stained with anti-FLAG and a secondary anti-mouse antibody, labeled with Cy2 (green). HDAC1 was labeled with Cy3 (red). Nuclei were counterstained with DAPI (blue).