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Figure 3


Fig. 3. Xevl regulates epithelial morphology in the otic vesicle. Confocal images of whole-mount preparations showing (A,A') {alpha}-catenin, (B,B') vinculin, and (C,C') occludin immunostaining in the otic vesicle. Uninjected otic vesicles at stage 35 comprise a hollow sphere of columnar epithelial cells. (A-C) The otic epithelium exhibits apical-basal polarity with enhanced {alpha}-catenin, vinculin and occludin localization at apical junctions (arrows). (D-F) Xevl depletion results in a smaller otic vesicle, disruption of columnar morphology, and loss of apical localization of (D,D') {alpha}-catenin, (E,E') vinculin and (F,F') occludin. Arrows in A-F mark regions shown at higher magnification in A'-F'. Bars, 50 µm.