Fig. 5. Attenuation of Abcg2 expression adversely affects pan neural and retinal progenitor properties in late retinal progenitors. (A-I) There was a significant decrease in the proportion of BrdU⁺ cells expressing the neural progenitor marker, Nestin (A-D,I) and retinal marker, Pax6 (E-H,I) in pSuper-ABCG2_siRNA retrovirus-transduced neurospheres, compared with controls. (J) RT-PCR analysis revealed a decrease in levels of transcripts corresponding to Pax6, Nestin and Sox2 in pSuper-ABCG2_siRNA-transduced neurospheres compared with controls, corroborating the immunocytochemical analysis. Data are expressed as the mean ± s.e.m. of triplicate cultures from two different experiments. Magnification, 200x.