Fig. 8. Ectopic Abcg2 expression influences Mash1 and cell cycle regulators expression. SP and NSP cells were enriched by Hoechst dye efflux assay from neurospheres, transduced with G1-ABCG2 retrovirus or empty retrovirus, followed by examination of expression of transcripts corresponding to a proneural gene (Mash1), cell cycle regulators (Ccnd1, Ki67, p27Kip1) and pan neural marker (Map2). Levels of Mash1, CyclinD and Ki67 transcripts were increased in SP cells obtained from ABCG2 retrovirus-transduced neurospheres compared with those from control neurosheres. Whereas levels of p27Kip1 and Map2 transcripts were detected only in NSP cells and their levels decreased in those transduced with ABCG2 retrovirus, compared with those in controls.