JCS -- Moore et al. 120 (15): 2683 Figure IG1

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Fig. 1. Developmental expression and localization of endogenous centaurin ${alpha}$ 1 in the rodent brain. (A) Immunoblot analysis of centaurin ${alpha}$ 1 protein from lysates (25 µg total lysate per lane) from whole brain at different developmental stages from E10 to P42 (=adult). (B) Immunoblot analysis of centaurin ${alpha}$ 1 and synaptic markers in fractionated P23 whole rat brain. H, whole brain homogenate; P2, crude microsomes and synaptosomes; S2, supernatant; Syn, purified synaptosomes; 20 µg of each fraction was loaded per lane. (C) Immunoblot analysis of centaurin ${alpha}$ 1 in the synaptosome fraction prepared from the developing brain. 10 µg of each fraction was loaded per lane. (D) Immunohistochemistry with anti-centaurin ${alpha}$ 1 antibody in hippocampus from P28 rat. DG, dentate gyrus.