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Figure 2


Fig. 2. Expression of endogenous centaurin {alpha}1 in primary cultured dissociated hippocampal neurons. (A) Immunoblot showing developmental expression of centaurin {alpha}1 and the specific synaptic proteins PDS95, spinophilin (Spino) and synaptotagmin (Syn) in primary cultured neurons at 3, 7, 14 and 21 DIV. 20 µg of total lysate was loaded per lane. (B) Indirect immunofluorescence shows endogenous centaurin {alpha}1 localization (red) and levels at 3, 7, 14 and 21 DIV. Bars, 10 µm.