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Figure 6


Fig. 6. Effects of overexpression of a GAP-inactive mutant centaurin {alpha}1 on dendritic differentiation. Hippocampal neurons were transfected after dissociation (0 DIV) and cultured for 3-14 DIV. (A) Fluorescence (GFP, green) and indirect immunofluorescence (beta-tubulin, red) were used to visualize transfected neurons and neuronal morphology. Expression of GFP control (left panels) compared with GFP plus Flag-R49K centaurin {alpha}1 (right panel). Boxed areas show a higher magnification. Bars, 20 um. (B) Quantification of the effects of overexpression of Flag-R49Kcentaurin {alpha}1 for 3 and 7 DIV compared with GFP control. The data was quantified from three independent experiments (n=50 cells at 3 DIV and 30 cells at 7 DIV). Data represent mean ± s.e. *P<0.05.