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Figure 7


Fig. 7. Effects of overexpression of wild-type and GAP-inactive centaurin {alpha}1 on dendritic protrusions in dissociated cultured hippocampal neurons. Dissociated hippocampal neurons were transfected at 1 DIV and cultured for 13 DIV. (A) Fluorescence (GFP, green) and indirect immunofluorescence (spinophilin or neurabin, red) were used to visualize transfected neurons and morphological effects. Expression of GFP control (left panels) compared with GFP plus Flag-centaurin {alpha}1 (middle panel) and GFP plus Flag-R49Kcentaurin {alpha}1 (right panel). Bars, 10 µm. (B) Quantification of the number of dendritic protrusions per 10 µm dendrite length in neurons expressing GFP, GFP plus Flag-centaurin {alpha}1 and GFP plus Flag-R49K centaurin {alpha}1. Data represent mean ± s.e. *P<0.05.