Fig. 6. TC10 also antagonises NGF-induced N-WASP activation. (A) Irregularity indexes (average ± s.e.m.) of NGF-induced PC12 cells transfected with empty vector or expressing constitutively active HA-tagged TC10–Q67L (left). The western blot shows the similar expression levels of the constitutively active mutants of both GTPases used in this study, HA-tagged TC10–Q67L and Cdc42-G12V, relative to actin (right). (B) Representative images of N-WASP biosensor expressing PC12 cells co-expressing HA-tagged TC10-Q67L. Intensities of CFP (Donor), YFP (Acceptor) and the FRET ratio are shown (left) for representative cells; cumulative FRET ratios are shown for both conditions as in Fig. 4 (right). Bars, 5 µm (untreated cells); 10 µm (NGF-treated cells).