Fig. 7. Exo70-induced repression of peripheral N-WASP activity is restored by constitutively active Cdc42 but not by constitutively active TC10. (A,B) Representative images of N-WASP biosensor expressing PC12 cells co-expressing constitutively active HA-tagged TC10-Q69L and FLAG-tagged Exo70 (A) or constitutively active HA-Cdc42-G12V and FLAG-Exo70 (B). Intensities of CFP (Donor), YFP (Acceptor) and the FRET ratio are shown (left) for representative cells; cumulative FRET ratios are shown for both conditions as in Fig. 4 (right). Bars, 5 µm (untreated cells), 10 µm (NGF-treated cells).