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Figure 3


Fig. 3. Inhibition of PI3K and SFK blocked OXO-mediated scattering of RIE1-{alpha}5 cells, but did not affect cell-cell contacts between RIE1-{alpha}5/1 cells. Cells were seeded onto 10% FBS-DMEM-H-coated glass coverslips. Once cells formed monolayers, they were not pretreated or pretreated with LY294002 (LY, 20 µM), PP2 (10 µM), or PP3 (10 µM), 30 minutes before DMSO or 10 µM OXO treatment. Cells were processed for immunostaining against ZO1, 24 hours later, as described in Materials and Methods. Data shown are representative of three independent experiments.