Fig. 6. Recruitment of DNA repair factors to various types of DNA damage. (A) XPC-GFP accumulates most efficiently in areas damaged with 266 nm laser light. The presence of Hoechst 33342 causes slower diffusion of XPC thus retarding its recruitment to DNA damage. (B) GFP-XPA also accumulates most efficiently in areas damaged with 266 nm laser light. GFP-XPA responds to a very small extent to pulsed 800 nm irradiation and 405 nm irradiation combined with Hoechst 33342. (C) YFP-MDC1(BRCT) is recruited quicker and in higher numbers to damaged areas in cells irradiated with 405 nm combined with Hoechst 33342 than in pulsed 800 nm-irradiated cells. (D) Rad54-GFP has a delayed response to pulsed 800 nm irradiation but it accumulates to a larger extent to these damages than to 405 nm combined with Hoechst 33342 irradiation.