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Figure 4


Fig. 4. DAB-1 localisation in chc-1(RNAi) and apa-2(RNAi) oocytes. Deconvolved image stacks of oocytes derived from DH1033 hermaphrodites, showing localisation of YP170::GFP (A,D,G,J,M,P) and DAB-1 (B,E,H,K,N,Q). Wild-type oocytes (A-F) show the characteristic DAB-1 puncta. Knockdown of chc-1(RNAi) (G-L) leads to the almost complete loss of DAB-1 puncta, however DAB-1 remains associated with the membrane. Similar results were obtained in apa-2(RNAi) oocytes (M-R). All images were collected using identical camera settings. Bar, 5 µm.